None applicable
1. elucidation of the molecular basis for the role of bacterial toxins in the pathogenesis of staphylococcal infections and their control Staphylococcus aureus causes mastitis in cattle and pyogenic diseases in many livestock and poultry, and causes pyogenic diseases of various organs, food poisoning, and toxic shock syndrome in humans. We have analyzed the genetic diversity of enterotoxins (SEs) and toxic shock syndrome toxin (TSST-1), which are superantigenic toxins produced by Staphylococcus aureus, to understand the transmission of toxin genes and drug resistance genes between humans and animal species, toxin expression mechanisms in vivo and in the environment, toxin expression in staphylococcal infection We aim to elucidate the role of toxin in the establishment and persistent infection of staphylococci, and to develop novel vaccines to protect against infection. 2) Genetic characterization of foodborne pathogenic Staphylococcus aureus and analysis of the regulatory mechanism of toxin expression in foods Staphylococcal food poisoning is caused by the growth of Staphylococcus aureus in foods, and human consumption of SEs produced as a result. We will identify the population of staphylococci causing food poisoning from among various staphylococci, analyze their genetic characteristics, clarify the regulatory mechanism of SEs production in food, and establish countermeasures to control food contamination. 3. 3. elucidate the mechanism of SEs emesis and molecular mechanism of food poisoning development We have been elucidating the mechanism of action of SEs using Suncks and common marmosets as animal models of emesis, and have revealed that mast cells in the intestinal submucosal tissue are the target cells of SEA. Currently, we are elucidating the mechanism of entry of SEs into intestinal cells, receptors on mast cells or neurons, and the molecular basis of emetic expression. 4. 4. biological characterization of novel staphylococcal enterotoxin-like toxins (SEls) and their risk assessment in food poisoning In recent years, novel toxins SEs and SEls have been discovered one after another, including our research group. In the future, we would like to further elucidate the epidemiological characteristics, biological properties, superantigen activity, and emesis-inducing activity of these novel toxins.
【学術論文】
・Ono, H, K., Hirose, S., Narita, K., Sugiyama, M., Asano, K., Hu, D.-L., Nakane A. Histamine release from intestinal mast cells induced by staphylococcal enterotoxin A (SEA) evokes vomiting reflex in common marmoset. PloS Pathogens. 21;15(5): e1007803. doi: 10.1371/journal.ppat.1007803. 2019.
・Fang, R., Cui, J., Cui, T., Guo, H., Ono, H.K., Park, C.H., Okamura, M., Nakane, A. and Hu, D.-L. Staphylococcal enterotoxin C is an important virulence factor for mastitis. Toxins (Basel). doi: 10.3390/toxins11030141. 11(1): 141. 2019.
・Narita, K., Hu, D.-L., Asano, K. Nakane, A. IL-10 produced by mutant toxic shock syndrome toxin-1 vaccine-induced memory T cells downregulates IL-17 production and abrogates the protective effect against Staphylococcus aureus infection. Infect. Immun. doi:10.1128/IAI.00494-19. 2019.
・Feng, S., Chen, T., Lei, G., Hou, F., Jiang, J., Huang, Q., Peng, Y., Ye, C., Hu D.-L., Fang, R. Absent in melanoma 2 inflammasome is required for host defence against Streptococcus pneumoniae infection. Innate Immunol. 25 (7): 412-419. 2019. doi: 10.1177/1753425919860252. 2019.
・Chen, T., Jiang, J., Ye, C., Xie, J., Chen, X., Xu, D., Zeng, Z., Peng, Y., Hu, D.-L. Fang, R. Genotypic characterization and antimicrobial resistance profile of Salmonella isolated from chicken, pork and the environment at abattoirs and supermarkets in Chongqing, China. BMC Veterinary Research. 18;15(1):456. doi: 10.1186/s12917-019-2202-4, 2019.
・Ono, H. K., Hachiya, N., Suzuki, Y., Naito, I., Hirose, S., Asano, K., Omoe, K., Nakane, A. and Hu, D.-L., Development of An Immunoassay for Detection of Staphylococcal Enterotoxin-like J, A Non-Characterized Toxin. Toxins (Basel). 10: E485: 2018.
・Yu, L., Wang, S., Guo, Z., Liu, H., Sun, D., Yan, G., Hu, D.-L., Du, R., Feng, X., Han, W., Gu, J., Sun, C. and Lei, L-C. A guard-killer phage cocktail effectively lyses the host and inhibits the development of phage-resistantstrains of Escherichia coli. Appl. Microbiol. Biotechnol. 102 (2): 971-983, 2018.
・Ono, H. K., Hirose, S., Naito, I., Sato’o, Y., Asano, K., Hu, D.-L., Omoe, K. and Nakane, A. Emetic activity of staphylococcal enterotoxins, SEK, SEL, SEM, SEN and SEO, in house musk shrew (Suncus murinus). Microbiol. Immunol. 61(1): 12-16. 2017.
・Hu, D.-L., Ono, H. K., Isayama, S., Okada, R., Okamura, M., Lei, L.C., Liu, ZS., Zhang, X.C., Liu, M.Y., Cui, J.C. and Nakane, A. Biological characteristics of staphylococcal enterotoxin Q and its potential risk for food poisoning. J. Appl. Microbiol. 122: 1672-1679. 2017.
・Hirose, S., Ono, H., Omoe, K., Hu, D.-L., Asano, K., Yamamoto, Y. and Nakane, A. Goblet cells are involved in translocation of staphylococcal enterotoxin A in the intestinal tissue of house musk shrew (Suncus murinus). J. Appl. Microbiol. 120(3): 781-789. 2016.
・Asano, K., Sashinami, H., Osanai, A., Hirose, S., Ono, H.K., Narita, K., Hu, D.-L., Nakane, A. Passive immunization with anti-ActA and anti-listeriolysin O antibodies protects against Listeria monocytogenes infection in mice. Scientific Reports. 6:39628 | DOI: 10.1038/srep39628. 2016.
・Narita, K., Hu, D.-L., Asano, K. and Nakane, A. Vaccination with nontoxic mutant toxic shock syndrome toxin-1 induces IL-17-dependent protection against Staphylococcus aureus infection. FEMS Pathog. Dis. 73(4): 1-10. 2015.
・Ono, H.K., Sato'o, Y., Narita, K., Naito, I., Hirose, S., Hisatsune, J., Asano, K., Hu, D.-L., Omoe, K., Sugai, M. and Nakane, A. Identification and characterization of a novel staphylococcal emetic toxin. Appl. Environ. Microbiol. 81(20): 7034-7040. 2015.
【総説・その他】
・胡 東良、岡村雅史. ブドウ球菌エンテロトキシンの加熱および消化酵素に対する抵抗性. 防菌防黴. 43(9): 425-430. 2015.
・胡 東良、岡村雅史. ブドウ球菌エンテロトキシンに関する最新の知見―特に分子多様性と生物学的多機能性について―. 日食微誌，32(2): 87-94. 2015.
【著書・翻訳】
・胡 東良 (分担執筆), 伝染性コリーザ，鶏ブドウ球菌症, 鶏カンピロバクター症, 等, 明石博臣ら（編集）, 動物の感染症（第四版）, 東京, 近代出版, p.210-216, 2019.
・胡 東良 (分担執筆), C. スタフィロコッカス属（ブドウ球菌属），D. その他のグラム陽性菌, 日本獣医学会微生物学分科会（編）関崎勉ら（編集）, 獣医微生物学（第四版）, 東京, 文永堂出版, p.170-176, 2018.
・胡 東良 (分担執筆), 第６章, 球菌（葡萄球菌属、連球菌属、其他病原性球菌），胡 桂学（主編）, 獣医微生物学, 北京, 中国農業大学出版社, p.82-99, 2018.
・Hu, D.-L. et al. Chapter 3. Staphylococcus aureus enterotoxins. Edited by Fetsch, A., Staphylococcus aureus. London. United Kingdom. Elsevier Academic Press. p. 39-56. 2017.
・胡 東良 (分担執筆), 魚類免疫学, 韓 文瑜・雷 連成（主編）, 高級動物免疫学 (Advanced Veterinary Immunology), 北京, 科学出版社, p.358-369, 2016.
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0176-24-9451 (Hu, direct), 0176-23-4371 ext. 487 (Ono)
